Development of new methods to study DNA methyltransferase function and protein interactions in living cells [Elektronische Ressource] / vorgelegt von Kourosh Zolghadr

Development of new methods to study DNA methyltransferase function and protein interactions in living cells Kourosh Zolghadr Dissertation an der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Kourosh Zolghadr aus Teheran München, den 18.12.2007 Erstgutachter: Prof. Dr. Heinrich Leonhardt Zweitgutachter: Prof. Dr. Harry MacWilliams Tag der mündlichen Prüfung: 19.03.2008 INDEX SUMMARY .............................................................................................................................................3 CHAPTER I. INTRODUCTION................................................................................................................5 I.1. How to detect protein interactions?....................................................................................................6 I.1.1. Antibodies and derivates thereof to identify proteins ...................................................................6 I.1.2. Biochemical methods to study protein interactions .....................................................................8 I.1.3. Genetic yeast two-hybrid screens to detect protein-protein interactions ....................................9 I.1.4. Fluorescently labeled proteins and applications in living cells..................................................10 I.2.