Development of a spectrally resolved fluorescence 3D for lifetime imaging microscopy precision measurements in the nanometer range [Elektronische Ressource] / vorgelegt von John Wallis

INAUGURAL-DISSERTATION Zur Erlangung der Doktorwürde Der Naturwissenschaftlich-Mathematischen Gesamtfakultät Der Ruprecht-Karls-Universität Heidelberg Vorgelegt von Diplom-Mathematiker John Wallis Aus Aurora, Colorado Tag der Mündlichen Prüfung: 11. Juli 2003 DEVELOPMENT OF A SPECTRALLY-RESOLVED FLUORESCENCE 3D FOR LIFETIME IMAGING MICROSCROPY PRECISION MEASUREMENTS IN THE NANOMETER RANGE Gutachter: Prof. Dr. Jürgen Wolfrum Prof. Dr. Joachim Pius SpatzTo my wife ii ACKNOWLEDGMENTS I would like to thank my wife for her endless moral support during this work. I would also like to thank my parents, my wife’s parents, and my sons, Charles and Philip. I would also like to thank all the people at the chemical and numerical sections of the University of Heidelberg for their support. iii SUMMARY This work developed a program to monitor and collect florescence photons from single molecules in three-dimensional domains with high precision and developed tools for viewing such data. The resulting scanned images reveals better understanding of the interplay between the molecules. These images also help the researcher find areas of interest and he can quickly measure in more detail these areas.