Development of a screening system for the determination of compounds in urine by automated on-line extraction HPLC-DAD for toxicological analysis [Elektronische Ressource] / von Lena Schönberg

Development of a Screening System for the Determination of Compounds in Urine by Automated On-line Extraction HPLC-DAD for Toxicological Analysis Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) Vorgelegt der Naturwissenschaftlichen Fakultät I / Biowissenschaften der Martin-Luther-Universität Halle-Wittenberg von Lena Schönberg geb. am 08.09.1976 in Tauberbischofsheim Gutachter: 1. Prof. Dr. Ch. Kloft 2. PD. Dr. D. Lampe 3. Prof. Dr. P. Imming Halle (Saale), 28.01.2008 urn:nbn:de:gbv:3-000013146[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000013146]Abstract ii Abstract Screening for a wide range of xenobiotics in biological samples is an important task for clinical toxicological and forensic laboratories. The aim of this thesis was to develop a fully automated on-line extraction HPLC-DAD screening method for the determination of compounds in urine with access to a commercially available UV spectra library with approximately 2600 reference spectra for compound identification. The method should allow simple analysis of those compounds that are difficult to detect in plasma due to short half-lives in blood (e.g. alkaloids) and therefore usually require work- and/or cost-intensive analytical methods. Furthermore, the method should be used for drugs of abuse (DOA) confirmation screening in urine.