Development and application of a novel in vivo EGFP based V(D)J recombination assay [Elektronische Ressource] / Zheng Xiao

Department of Transfusion MedicineTutor: Prof. Dr. B. Kubanek / Dr. K. SchwarzDevelopment and Application of a Novelin vivo EGFP Based V(D)J Recombination AssayDissertationForObtaining A Doctoral DegreeOf Human BiologyAt The Faculty Of Medicine,University Of UlmPresented byZheng Xiaofrom Wuhan, P.R. ChinaUlm, October, 18, 20021Contents1. Abbreviations 52. Introduction 82.1. The Principles of V(D)J Recombination 112.2. Components of the V(D)J Recombination Machinery 142.2.1. Lymphocyte Specific Factors 16 2.2.1.1. Recombinase Activating Genes 1 and 2 (RAG-1 and -2) 162.2.1.1.1. Structure and Function 162.2.1.1.2. Mutation Analysis 182.2.1.2. Terminal Deoxynucleotidyl Transferase (TdT) 192.2.2.