Crystallisation and structure solution of the c-ring of F_1tnoF_1tn1 ATP synthase from spinach chloroplasts [Elektronische Ressource] / vorgelegt von Melanie Vollmar

Crystallisation and Structure Solutionof the c-Ring of FF ATP Synthaseo 1from Spinach ChloroplastsInaugural-DissertationzurErlangung des Doktorgradesder Mathematisch-Naturwissenschaftlichen Fakultätder Heinrich-Heine-Universität Düsseldorfvorgelegt vonMelanie Vollmaraus KapsweyerJuli 2009Aus dem Institut für biochemische Pflanzenphysiologieder Heinrich-Heine-Universität DüsseldorfGedruckt mit der Genehmigungder Mathematisch-Naturwissenschaftlichen Fakultätder Heinrich-Heine-Universität DüsseldorfReferent: Prof. Dr. G. GrothKoreferent: Prof. Dr. L. SchmittTag der mündlichen Prüfung: 17.09.2009Meiner MutterSummaryATP synthase from spinach belongs to a group of essential enzymes which produce most of the cell's ATP supply. The energy stored in light is used in photosynthetic active complexes t o producean outward pointing electron/proton gradient across the thylakoid membrane of chloroplast s. Thisenergisation of the membrane is a prerequisite for actiFve AFTP synthase. ATP synthase pumps o 1protons back across the thylakoid membrane into the chloroplast. During this translocation process,the electrical energy of the gradient is transformed into the mechanical energy of a rot ation. Thisrotation takes place in the membrane part F and is translated via a stalk into a membrane ass ociatedopart F which carries the catalytic active sites.