Construction of a BAC-PAC contig of SSC 6q1.2 and comparative analysis of this genome region [Elektronische Ressource] / von Flávia Martins-Weß (geborene de Sá Martins)

Construction of a BAC/PAC contig of SSC 6q1.2 and comparative analysis of this genome region Vom Fachbereich Biologie der Universität Hannover zur Erlangung des Grades Doktorin der Naturwissenschaften Dr. rer. nat. genehmigte Dissertation von Flávia Martins-Weß (geborene de Sá Martins) geboren am 19.09.1975 in São Paulo (Brasilien) 2003 Referent: Univ.-Prof. Dr. Tosso Leeb Co-Referent: Univ.-Prof. Dr. Hans-Jörg Jacobsen 3. Gutachter: Univ.-Prof. Dr. Dr. Bertram Brenig Tag der Promotion: 28. November 2003 Dedicated to my parents and to Carsten Abstract Abstract The RYR1 gene region on the porcine chromosome 6q1.2 is known to harbour the locus for malignant hyperthermia (MH) or stress susceptibility. Porcine stress syndrome (PSS) has been responsible for economic losses in stress-susceptible pigs. These animals have increased muscling and lean meat content. So far it is unknown whether only the RYR1 mutation is responsible for the positive carcass traits in stress-susceptible pigs or if these complex growth traits are influenced by other closely linked genes on SSC 6q1.2. The purpose of this study was to construct a detailed physical map of the RYR1 gene region as an important resource for the DNA sequencing of the coding regions on the porcine chromosome 6q1.2.