Connecting the histone acetyltransferase complex SAS-I to the centromere in S. cerevisiae [Elektronische Ressource] / von Stefanie Seitz

Connecting the histone acetyltransferase complex SAS-I to the centromere in S. cerevisiae DISSERTATION zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) im Fach Biologie eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt-Universität zu Berlin von Dipl. biol. Stefanie Seitz geb. 18.06.1975, Frankfurt/Main Präsident der Humboldt-Universität zu Berlin Prof. Dr. Jürgen Mlynek Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I Prof. Dr. Michael Linscheid Gutachter/-innen: 1.- Prof. Dr. Ann Ehrenhofer-Murray 2.- Prof. Dr. Harald Saumweber 3.- Prof. Dr. Francis Stewart Tag der mündlichen Prüfung: 20.10.2004 Abstract The essential histone H3 variant Cse4 plays a crucial role at the centromere in S. cerevisiae, where it replaces histone H3 in that it assembles centromere specific (Cse4-H4)2 tetrameres. We found in our study that the histone H3 variant was able to interact over its unique N-Terminus with two subunits of the histone acetyltransferase complex SAS-I: Sas2 and Sas4. Mutations within the acetyl-CoA binding site (HAT domain) or the zink-finger of Sas2 disrupted the binding to Cse4, although an indirect interaction was found with co-immunoprecipitation experiments.