Comprehensive metabolite analysis in Chlamydomonas reinhardtii [Elektronische Ressource] : method development and application to the study of environmental and genetic perturbations / von Christian Bölling

Institut für Biochemie und Biologie Max-Planck-Institut für Molekulare Pflanzenphysiologie Arbeitsgruppe Metabolomic Analysis Comprehensive metabolite analysis in Chlamydomonas reinhardtii Method development and application to the study of environmental and genetic perturbations Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) in der Wissenschaftsdisziplin Biochemie der Pflanzen eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät der Universität Potsdam von Christian Bölling Potsdam, den 01.09.2006 Summary This study introduces a method for multiparallel analysis of small organic compounds in the unicellular green alga Chlamydomonas reinhardtii, one of the premier model organisms in cell biology. With the recent completion of the Chlamydomonas genome project, a systems biology perspective to develop an understanding of how the numerous components in a living system interact to comprise a functioning whole comes into focus for this model organism too. The comprehensive study of the changes of metabolite composition, or metabolomics, in response to environmental, genetic or developmental signals is an important complement of other functional genomic techniques in the effort to develop an understanding of how genes, proteins and metabolites are all integrated into a seamless and dynamic network to sustain cellular functions.