Comparative analyses of the neurogenic capacity of human neuroprogenitor populations derived from neural and mesodermal tissue [Elektronische Ressource] / vorgelegt von Martina Wölfle, geb. Maisel

Abteilung für Neurologie Leiter: Prof. Dr. A. C. Ludolph Universität Ulm Comparative analyses of the neurogenic capacity of human neuroprogenitor populations derived from neural and mesodermal tissue Dissertation zur Erlangung des Doktorgrades Dr. hum. biol. der Medizinischen Fakultät der Universität Ulm vorgelegt von Martina Wölfle, geb. Maisel geboren in Böblingen Ulm im Mai 2007 Amtierender Dekan: Prof. Dr. Klaus-Michael Debatin 1.Gutachter: Prof. Dr. Alexander Storch 2.Gutachter: Prof. Dr. Tobias Böckers Tag der Promotion: 16. November 2007 Mach` immer was dein Herz dir sagt Meiner Familie Table of contents Table of contents 1 INTRODUCTION 1 1.1 NEURAL STEM OR PROGENITOR CELLS 2 1.2 SOURCES OF NEUROPROGENITORS 3 1.2.1 FETAL MAMMALIAN BRAIN 3 1.2.2 NEUROGENESIS IN THE ADULT MAMMALIAN BRAIN 5 1.2.3 MESODERMAL STEM CELLS 7 1.2.4 NEUROECTODERMAL CONVERSION OF MESODERMAL STEM CELLS 8 1.3 DIFFERENTIATION CAPACITY OF NEUROPROGENITORS 9 1.3.1 DIFFERENTIATION POTENTIAL OF FETAL NEURAL PROGENITOR CELLS 9 1.3.2 DIFFERENTIATION CAPACITY OF ADULT NEUROPROGENITOR CELLS 10 1.4 AIM OF THIS THESIS 11 2 MATERIALS AND METHODS 13 2.1 ADULT HUMAN HIPPOCAMPAL NEURAL PROGENITOR CELL ISOLATION 13 2.2 FETAL HUMAN CORTICAL NEURAL PROGENITOR CELL ISOLATION 14 2.