Cloning and characterization of two glycosidases from the acidothermophile Alicyclobacillus acidocaldarius ATCC27009 [Elektronische Ressource] / von Kelvin Eckert

Cloning and characterization of two glycosidasesfrom the acidothermophile Alicyclobacillusacidocaldarius ATCC27009D I S S E R T A T I O Nzur Erlangung des akademischen Gradesdoctor rerum naturalium(dr. rer. nat.)im Fach Biologieeingereicht an derMathematisch-Naturwissenschaftlichen Fakult¨at IHumboldt-Universitat¨ zu BerlinvonHerr Dipl.-Biol. Kelvin Eckertgeboren am 18.12.1971 in BerlinPr¨asident der Humboldt-Universit¨at zu Berlin:Prof. Dr. Jurg¨ en MlynekDekan der Mathematisch-Naturwissenschaftlichen Fakult¨at I:Prof. Dr. Michael LinscheidGutachter:1. Prof. Dr. rer. nat. Erwin Schneider2. Prof. Dr. rer. nat. Rainer Borriss3. Prof. Dr. rer. nat. Evert Bakkereingereicht am: 17. September 2003Tag der mundlic¨ hen Prufung:¨ 6. Februar 2004Part of this work has been published in the following papers:Eckert, K. and Schneider, E. (2003). A thermoacidophilic endoglu-canase (CelB) from Alicyclobacillus acidocaldarius displays a high sequencesimilaritytoarabinofuranosidasesbelongingtofamily51ofglycosidehydro-lases. Eur. J. Biochem. 270: 3593-3602.Eckert,K.,Ernst,H.A.,Schneider,E.,Larsen,S.andLoLeggio,L.(2003). Crystallization and preliminary X-ray analysis of Alicyclobacillusacidocaldarius endoglucanase CelA. Acta Crystallogr. D. Biol. Crystallogr.59: 139-141.Eckert, K., Zielinski, F., Lo Leggio, L. and Schneider, E. (2002).