Characterization of the new synaptic protein LAPSER1 [Elektronische Ressource] / Michael Joachim Schmeißer

Universität Ulm Institut für Anatomie und Zellbiologie Amtierender Direktor: Prof. Dr. med. Tobias M. Böckers Characterization of the new synaptic protein LAPSER1 Dissertation zur Erlangung des Doktorgrades der Medizin der Medizinischen Fakultät der Universität Ulm Michael Joachim Schmeißer aus Kempten (Allgäu) 2009 Amtierender Dekan: Prof. Dr. rer. nat. Thomas Wirth 1. Berichterstatter: Prof. Dr. med. Tobias M. Böckers 2. Berichterstatter: Prof. Dr. med. Hayrettin Tumani Tag der Promotion: 28.10.2010 Für meine lieben Eltern und meinen Opa Kurt, der leider 2008 von uns ging. Table of contents Table of contents List of abbreviations III 1. Introduction 11.1 Neurons 11.2 Synapses 11.3 Synaptogenesis 31.4 Synaptic plasticity 41.5 The presynaptic active zone 51.6 The postsynaptic density 61.7 Goals of this work 15 2. Materials & Methods 162.1 Materials 162.2 Methods of microbiology 272.3 Methods of molecular biology 302.4 Methods of eukaryotic cell culture 342.5 Methods of protein biochemistry 382.6 Analysis of mRNA and protein localization 452.7 Analysis of protein-protein interactions 50 3. Results 543.1 Genomic and protein structure of LAPSER1 543.2 LAPSER1 belongs to the “Fezzin” protein family 563.3 Localization of LAPSER1 mRNA 573.4 Localization of the LAPSER1 protein 583.