Characterization of the microbial community in a biogas reactor supplied with organic residues [Elektronische Ressource] / Frank Bengelsdorf

Characterization of the microbial community in a biogas reactor supplied with organic residues Dissertation Submitted for the fulfillment of the requirements for the doctoral degree Dr. rer. nat at the Faculty of Natural Sciences, University of Ulm By Frank Robert Bengelsdorf from Wismar 2011 The current study was prepared at the Institute of Microbiology and Biotechnology, University of Ulm. Dekan: Prof. Dr. Axel Groß 1. Reviewer: Prof. Dr. Peter Dürre 2. Reviewer: Prof. Dr. Marian Kazda Graduation date: 15-Dec.-2011 Contents I Contents Abbreviations ........................................................................................................................... IV 1 Introduction ............................. 1 2 Materials and Methods ........................................................................................................... 5 2.1 Biogas plant .......................................................................................................................... 5 2.2 Sampling ............................... 6 2.2.1 Standard sampling for genomic DNA extraction ............................................................ 6 2.2.2 Sampling for 454 Pyrosequencing .................................................. 7 2.2.3 Sampling for microscopy ................................ 8 2.