Characterization and modification of genetically encoded indicators to monitor neural activity in Drosophila melanogaster [Elektronische Ressource] / Alexandra Ihring

CHARACTERIZATION AND MODIFICATION OF GENETICALLY ENCODED INDICATORS TO MONITOR NEURAL ACTIVITY IN DROSOPHILA MELANOGASTER Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften an der Fakultät für Biologie der Ludwig-Maximilians-Universität München Alexandra Ihring München, 2006 Erstgutachter: Prof. Dr. Alexander Borst Zweitgutachter: Prof. Dr. Rainer Uhl Tag der mündlichen Prüfung: 18.Juli 2006 TABLE OF CONTENTS Table of Contents Table of Contents…………………………………………………..……….…I Table of Figures……………………………………………….….……..…….V Abbreviations……………………………………………………………...…IX 1 Abstract...............................................................................................1 2 Introduction........................................................................................3 2.1 General..........................................................................................................3 2.2 Green Fluorescencent Protein ....................................................................5 2.3 Fluorescence and FRET ..............................................................................7 2.4 Genetically Encoded Indicators................................................................10 2.4.1 Voltage Sensors...................................................................................10 2.4.