Characterisation of components and mechanisms involved in redox regulation of protein import into chloroplasts [Elektronische Ressource] / vorgelegt von Anna Stengel

Characterisation of components and mechanisms involved in redox-regulation of protein import into chloroplasts Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften an der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Anna Stengel München, Mai 2009 Erstgutachter: Prof. Jürgen Soll Zweitgutachter: Prof. Jörg Nickelsen Tag der mündlichen Prüfung: 28. Mai 2009 Table of contents Sumary 1 Zusamenfasung 3 Introduction 5 The origin of chloroplasts and a general description of protein import 5 The Toc omplex 7 The Tic coplex 8 Alternative import pathways 11 Evolution of the import machineries 12 Regulation pathways of protein import at the outer an inner envelope 14 Redox signals in the chloroplast 15 The thioredoxin system Redox-mediated regulation of protein import 17 Aim of this study 19 Results 20 Chapter 1: Tic62 - a protein family from metabolism to protein translocation. 22 Chapter 2: Tic62 - redox-regulated translocon composition and dynamics.