Cellular mechanisms regulating the enzymatic activities of cGMP-producing natriuretic peptide receptors [Elektronische Ressource] : studies in Mus musculus cell lines / presented by Lourdes Cortes-Dericks

Cellular Mechanisms Regulating the Enzymatic Activities of cGMP-Producing Natriuretic Peptide Receptors: Studies in Mus musculus Cell Lines Dissertation for the attainment of Doctoral Degree from the Faculty of Biology, University of Hamburg Presented by Lourdes Cortes-Dericks Hamburg April 2005 “One never notes what has been done; One can only see what remains to be done…” Marie Curie, 1894 Table of Contents 3 Table of Contents Abstract 7 1 Introduction 9 1.1 Natriuretic peptides 9 1.1.1 Structure and biosynthesis of natriuretic peptides 9 1.1.2 Proposed roles for natriuretic peptides in testicular Leydig cells and gonadotrophs of the pituitary gland 10 1.2 The Natriuretic Peptide Receptors 10 1.2.1 Structure of cGMP-generating natriuretic peptide receptors 12 1.2.1.1 Extracellular Domain 12 1.2.1.2 Transmembrane domain 13 1.2.1.3 Kinase homology domain 13 1.2.1.4 Hinge Region 13 1.2.1.5 Guanylyl cyclase catalytic domain 14 1.3 cGMP signalling 14 1.3.1 cGMP-generating systems 15 1.3.2 cGMP-target proteins 16 1.3.2.1 cGMP-dependent protein kinase 16 1.3.2.2 cGMP-regulated PDEs 17 1.3.2.3 cGMP-regulated ion channel 17 1.3.2.4 cAMP-dependent protein kinase (PKA) 18 1.4 Regulation of cGMP-generating natriuretic peptide receptors 18 1.4.1 Regulation by Phosphorylation 18 1.4.2 Homologous Desensitization 20 1.4.3 Heterologous Desensitization 21 1.5 Lysophosphatidic acid 23 1.5.