cDNA library construction towards the identification of the ipecac alkaloid biosynthesis genes in tissue cultures of Psychotria ipecacuanha [Elektronische Ressource] : Preliminary characterization of the first isolated enzyme, ipecoside β-D-glucosidase / von Alfonso Lara Quesada

Martin-Luther-Universität Halle-Wittenberg Fachbereich Biochemie/Biotechnologie cDNA library construction towards the identification of the ipecac alkaloid biosynthesis genes in tissue cultures of Psychotria ipecacuanha: Preliminary characterization of the first isolated enzyme, ipecoside β-D-glucosidase Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) vorgelegt der Mathematisch-Naturwissenschaftlich-Technischen Fakultät (mathematisch-naturwissenschaftlicher Bereich) der Martin-Luther-Universität Halle-Wittenberg von Herrn Alfonso Lara Quesada geb. am 23.03.1974 in Costa Rica Gutachter 1. Prof. Dr. T.M. Kutchan 2. Prof. Dr. J. Stöckigt 3. Prof. Dr. B. Dräger Halle (Saale), 24. Mai 2007 urn:nbn:de:gbv:3-000011846[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000011846]Content 1. Introduction 1 1.1. Psychotria ipecacuanha 1 1.2. Tissue culture and main alkaloid contains 2 1.3. Alkaloids 3 1.4. Monoterpenoid indole alkaloid biosynthesis 5 1.5. Monoterpenoid isoquinoline (ipecac) alkaloid biosynthesis 6 1.6. β-Glucosidases 9 1.6.1. Glycoside hydrolases 9 1.6.2. β-D-Glucosidases 10 1.7. The molecular biology in the secondary metabolism 12 1.7.1. Homologous sequences and degenerate primers 12 1.7.2. Expressed sequence tags (EST) 14 1.8.