Cap-dependence of the poly(A)-specific ribonuclease PARN [Elektronische Ressource] / von Eva Dehlin

Cap-dependence of the Poly(A)-specific Ribonuclease PARN Dissertationsschrift zur Erlangung des adakemischen Grades Doktor der Naturwissenschaften -Dr. rer. Nat.- vorgelegt der Mathematisch-Naturwissenschaftlich-Technischen Fakultät der Martin-Luther-Universität Halle-Wittenberg von Eva Dehlin Datum der Verteidigung: 20. Februar 2004 Gutachter: Prof. E. WahleProf. M. Worminton Dr. Simon Morleyurn:nbn:de:gbv:3-000007155[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000007155] TABLE OF CONTENTS 1. INTRODUCTION 1 1.1 The major mRNA degradation pathways. 1 1.2 Nonsense-mediated decay (NMD). 5 1.3 Nonstop-mediated decay (NSD). 6 1.4 cis-elements and trans-acting factors controlling mRNA in somatic cells. 6 1.5 Translation. 9 1.6 Deadenylation and translation. 12 1.7 Deadenylating nucleases. 14 1.8 Aim of the thesis. 18 2. MATERIALS AND METHODS 20 2.1 Materials 20 2.1.1 Bacterial strains and cell lines. 2.1.2 Culture media 2.1.3 Proteins and enzymes. 21 2.1.4 Antibodies. 22 2.1.5 Nucleotides and Nucleic Acids. 22 2.1.6 Plasmids vectors. 22 2.1.7 Oligonucleotides. 23 2.1.8 Kits. 23 2.1.9 Column materials. 24 2.1.10 Chemicals 24 2.1.11 Miscellaneous. 25 2.2 Methods 25 2.2.1 Standard methods. 2.2.2 DNA methods.