Biosynthesis of lignans [Elektronische Ressource] / von Elisabeth Fuß

Biosynthesis of Lignans Habilitationsschrift zur Erlangung der Habilitation im Fach Biologie der Pflanzen an der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität, Düsseldorf von Elisabeth Fuß Januar 2007 E. Fuß Lignan Biosynthesis Table of Contents page 1. Zusammenfassung 3 2. General Introduction 10 2.1. Lignans and their function in plants and for human health 10 2.2. Biosynthesis of lignans 12 3. Lignans in in vitro cultures of Linum species 15 3.1. Introduction 16 3.2. Cell cultures 17 3.2.1. Enzymes in cell cultures of Linum species (biochemistry and molecular biology) 18 3.2.2. Differential accumulation of lignans due to genotypic variations 23 3.2.3. Elicitation 26 3.3. Conclusion 30 4. Genetic Manipulation of the lignan biosynthesis in Linum species 31 4.1. Introduction 31 4.2. Agrobacterium rhizogenes-mediated transformation of Linum species 32 4.3. Genetic manipulation of the lignan biosynthesis in hairy roots 37 4.4. Conclusion 40 5. Stereospecificity in lignan biosynthesis: Pinoresinol-lariciresinol reductase 41 5.1. Introduction 41 5.2. Enantiospecificity in lignan biosynthesis of Linum species 43 5.3. Molecular basis for different enantiospecificities of PLRs 47 5.4. Evolution of PLRs and their enantiospecificity 49 5.5. Conclusion 54 6. Outlook 55 7. References 56 8.