Assessing the role of sCYLD and Bcl-3 in immune regulation [Elektronische Ressource] / vorgelegt von Joumana Masri

Assessing the role of sCYLD and Bcl-3 in immune regulation Dissertation Zur Erlangung des Grades “Doktor der Naturwissenschaften” am Fachbereich Biologie der Johannes Gutenberg-Universität in Mainz vorgelegt von Joumana Masri aus Köln Mainz 2010 Dekan: Berichterstatter 1: Berichterstatter 2: Tag der mündlichen Prüfung: Table of Contents 1   Introduction ......................................................................................... 1  1.1   The NF-κB signaling pathway.......................................1  1.2   Regulation of the NF-κB pathway by ubiquitin signaling ...........................2  1.3   CYLD................................................................................3  1.4   The IκB -like molecule Bcl-3...........5  1.5   The role of NF-κB signaling in cells of the immune system ......................6  1.5.1   B lymphocytes..................................................................................................6  1.5.2   T lymphocytes..6  1.5.3   Dendritic cells (DCs).........................8  1.5.4   Macrophages....9  2   Specific aims of this thesis .............................................................. 11  3   Materials and Methods...................................... 13 3.1   Chemicals and biological material.............................13  3.2   Molecular biology.........................