Analysis of the mammalian pre-mRNA 3' end processing cleavage complex and attempts of its reconstitution [Elektronische Ressource] / von Anne Ulrika Knoth

Analysis of the mammalian pre-mRNA 3’ end processing cleavage complex and attempts of its reconstitution Dissertation zur Erlangung des akademischen Grades Doctor rerum naturalium (Dr. rer. nat.) vorgelegt der Naturwissenschaftlichen Fakultät I Biowissenschaften der Martin-Luther-Universität Halle-Wittenberg von Frau Anne Ulrika Knoth geb. am 04.03.1979 in Halle (Saale) Gutachter: 1. Prof. Dr. E. Wahle 2. Prof. Dr. W. Keller 3. Prof. Dr. S.-E. Behrens Halle (Saale), den 09.06.2008 urn:nbn:de:gbv:3-000013658[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000013658] Success is the ability to go from one failure to another with no loss of enthusiasm. (Winston Churchill) Table of contents Page Table of contents I Abbreviations V 1. Introduction 1 1.1 mRNA maturation 1 1.2 Sequence elements in mRNA 3’ end processing 3 1.3 Proteins involved in mRNA 3’ end processing 5 1.3.1 Cleavage stimulation factor, CstF 6 1.3.2 Cleavage factor I , CF I 7 m m 1.3.3 Cleavage factor II , CF II 8 m m 1.3.4 Poly(A) polymerase, PAP 9 1.3.5 Cleavage and polyadenylation specificity factor, CPSF 10 1.4 Reconstitution of cleavage activity 12 1.5 Aim of this thesis 12 2. Results 14 2.1 Purification of the pre-mRNA 3’ end processing cleavage complex via 15 immobilised pre-mRNA 2.1.