Analysis of the factor XII-driven contact system activation in vivo [Elektronische Ressource] = Charakterisierung der Faktor-XII-vermittelten Aktivierung des Kontaktsystems in vivo / submitted by Felicitas Müller

Analysis of the factor XII-driven contact system activation in vivo Charakterisierung der Faktor XII-vermittelten Aktivierung des Kontaktsystems in vivo Doctoral thesis for a doctoral degree at the Graduate School of Life Sciences, Julius-Maximilians-Universität Würzburg, Section Biomedicine submitted by Felicitas Müller from Würzburg Würzburg, 2009 Submitted on: …………………………………………………………..…….. Members of the Promotionscommittee: Chairperson: Prof. Dr. Thomas Müller Primary Supervisor: Prof. Dr. Dr. Thomas Renné Supervisor (Second): Prof. Dr. Utz Fischer Supervisor (Third): Prof. Dr. Wolfdieter Schenk Date of Public Defence: …………………………………………….………… Date of Receipt of Certificates: ………………………………………………. DEDICATED TO MY FAMILYTABLE OF CONTENTS TABLE OF CONTENTS 1 SUMMARY 1 2 ZUSAMMENFASSUNG 2 3 INTRODUCTION 3 3.1 The contact system 3 3.2 Coagulation factor XII (Hageman factor) 4 3.3 The kallikrein-kinin system 7 3.4 The intrinsic pathway of coagulation 10 3.5 Activation of coagulation factor XII 15 3.6 Polyphosphate 16 3.7 Aim of this study 18 4 MATERIAL AND METHODS 19 4.1 Material 19 4.1.1 Chemicals 19 4.1.2 Enzymes, Antibodies, Proteins, Substrates, Inhibitors and Markers 19 4.1.3 Eukaryotic cells 21 4.1.