Analysis of sugar transport-related gene products expressed in developing seeds of Vicia faba and Hordeum vulgare [Elektronische Ressource] / von Qing Wang

Analysis of sugar transport-related gene products expressed indeveloping seeds of Vicia faba and Hordeum vulgareDissertationzur Erlangung des akademischen Gradesdoctor rerum naturalium (Dr. rer. nat. )vorgelegt derMathematisch-Naturwissenschaftlich-Technischen Fakultätder Martin-Luther-Universität Halle-Witterbergvon Frau Qing Wanggeb. am: 12. August 1968 in Peking, ChinaGutachterin bzw. Gutachter:1. Prof. Dr. U. Wobus2. Prof. Dr. Th. Boemer3. Prof. Dr. K. HumbeckHalle (Saale), den 09 September 2003urn:nbn:de:gbv:3-000005508[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000005508]PDF created with FinePrint pdfFactory trial version http://www.fineprint.comContentsDissertation 1Contents 2Acknowledgment 5Summary 6Zusammenfassung 9Abbreviations 121. Introduction 141.1 Sugar transport: loading and unloading the phloem 141.2 Sugar transporters 161.2.1 The Sucrose Transporters 171.2.2 The Hexose T 241.3 Seed development 271.3.1 Faba bean seed development 281.3.2 Barley seed development 30The aim of this work 312. Materials and Methods 332.1 Materials 332.1.1 Equipment 332.1.2 Enzyme and kits 332.1.3 Chemicals and other consumables 332.1.4 Plasmids 342.1.5 Plant material 342.1.6 Bacteria strains 352.1.7 Yeast strains 352.1.8 Oligouncleotides 352.2 Methods 372.2.1 Protein expression and Western blot analysis 372.2.1.1 Protein preparation from E.coli, yeast cells and plant tissues 372.2.1.2 Precipitation of protein 382.