Analysis of regulatory mechanisms governing aromatic compound degradation in Acinetobacter baylyi [Elektronische Ressource] / by Fenja Sabine Bleichrodt

Analysis of regulatory mechanisms governing aromatic compound degradation in Acinetobacter baylyi Dissertation Submitted for the fulfillment of the requirements for the doctoral degree Dr. rer. nat at the Faculty of Natural Sciences, University of Ulm By Fenja Sabine Bleichrodt from Berlin 2011 The current study was prepared at the Institute of Microbiology and Biotechnology, University of Ulm. Dekan: Prof. Dr. Thomas Wirth 1. Reviewer: apl. Prof. Dr. Ulrike Gerischer 2. Reviewer: Prof. Dr. Peter Dürre Tag der Promotion: 31.05.2011 Contents 1 Contents Abbreviations ............................................................................................................................................... 5 1 Introduction ......... 8 2 Materials and Methods ..................................................................................................................... 16 2.1 Plasmid and strain construction ................................................................................................... 16 2.2 Bacterial strains designed and used in this study ........ 18 2.3 Plasmids constructed and used in this study ................................................................................ 20 2.4 Primers used in this study ............................................ 21 2.5 Growth conditions ....................... 25 2.