Analysis of Chriz involved in Drosophila polytene chromosome structuring and binding [Elektronische Ressource] / von Miao Gan

Analysis of Chriz involved in Drosophilapolytene chromosome structuring and bindingDISSERTATIONzur Erlangung des akademischen Gradesdoctor rerum naturalium(Dr. rer. nat.)im Fach Biologieeingereicht an derMathematisch-NaturwissenschaftlichenFakultät IHumboldt-Universität zu BerlinvonFrau Miao Gangeboren am 23.06.1977 in Heilongjiang, P.R.ChinaPräsident der Humboldt-Universität zu Berlin:Prof. Dr. Dr. h.c. Christoph MarkschiesDekan der Mathematisch-NaturwissenschaftlichenFakultät I:Prof. Dr. Lutz-Helmut SchönGutachter:1. Prof. Dr. Achim Leutz2. Prof. Dr. Harald. Saumweber3. Prof. Dr. Ansgar Klebeseingereicht am: 27.01.2009Tag der mündlichen Prüfung: 27.05.2009AbstractDrosophila polytene chromosomes are compacted into a series of bands andinterbands. Z4 is a protein to keep this pattern of polytene chromosomes,since Z4 mutant larvae show a decompaction of chromosomes and a loss ofbanding pattern (Eggert et al., 2004). By coimmuno-precipitation, we iden-tified a chromodomain protein, which we named Chriz, for chromodomainprotein interacting with Z4 (Gortchakov et al., 2005).In my PhD thesis, I tested the interactions between the full length pro-teins and different fragments of Chriz and Z4 which showed that Chriz coulddirectly interact with Z4 in vivo. The interaction domains were mapped andit was determined that the N terminus of Z4 and the C terminus of Chriz aresufficient for mutual interaction.