Advanced schemes for surface plasmon resonance and plasmon-enhanced fluorescence biosensors [Elektronische Ressource] / vorgelegt von Chun-Jen Huang

Advanced Schemes for SurfacePlasmon Resonance andPlasmon-enhancedFluorescence BiosensorsDissertationzur Erlangung des Grades“Doktor der Naturwissenschafen”am Fachbereich Biologieder Johannes Gutenberg-Universitätin Mainzvorgelegt vonChun-Jen Huangaus Changhua, TAIWANMainz, November, 2010AbstractAdvanced optical biosensor platforms exploiting long range surface plasmons (LRSPs) andresponsive N-isopropylacrylamide (NIPAAm) hydrogel binding matrix for the detection ofprotein and bacterial pathogen analytes were carried out. LRSPs are optical waves thatoriginate from coupling of surface plasmons on the opposite sites of a thin metallic filmembedded between two dielectrics with similar refractive indices. LRSPs exhibit ordersof magnitude lower damping and more extended profile of field compared to regular sur-face plasmons (SPs). Their excitation is accompanied with narrow resonance and providesstronger enhancement of electromagnetic field intensity that can advance the sensitivityof surface plasmon resonance (SPR) and surface plasmon-enhanced fluorescence spec-troscopy (SPFS) biosensors. Firstly, we investigated thin gold layers deposited on fluo-ropolymer surface for the excitation of LRSPs. The study indicates that the morphological,optical and electrical properties of gold film can be changed by the surface energy of fluo-ropolymer and affect the performance of a SPFS biosensor.