Activation of apoptosis pathways by different classes of anticancer drugs [Elektronische Ressource] / Jiahao Liu

Research Laboratory of Children's Hospital, University of UlmChairman: Prof. Dr. Klaus-Michael DebatinActivation of apoptosis pathwaysby different classes of anticancer drugsDissertation for the applying for aDoctor Degree of Medicine (Dr. med.)Faculty of Medicine, University of UlmPresented by Jiahao LiuBorn in Hubei, P. R. China2001Amtierender Dekan: Prof. Dr. R. Marre1. Berichterstatter: Prof. Dr. K. M. Debatin2. Berichterstatter: Prof. Dr. Dr. Dr. A. GrünertTag der Promotion: 26. 10. 2001To my family:Chen Longgui &Liu ChangYD 1 ContentsContents 1Abbreviations 41. Introduction1.1. Apoptosis: definitions and mechanisms 6 1.1.1. Cell biology of apoptosis 61.1.2. Execution of programmed cell death by caspases 7 1.1.3. Two main pathways of apoptosis 81.2. Cytotoxic anticancer drugs and apoptosis 91.3. Aims and summary of the project 112. Materials and Methods2.1. Materials 14 2.1.1. Reagents and equipment for cell culture 14 2.1.2. Reagents and equipment for flow cytometric analysis 14 2.1.3. Reagents and equipment for western blot 152.1.4. Anticancer drugs 162.1.5. Antibodies 172.2. Methods 192.2.