A closer look at Schizosaccharomyces pombe interphase microtubule arrays [Elektronische Ressource] / vorgelegt von Hélio Roque

INAUGURAL – DISSERTATION zur Erlangung der Doktorwürde der Naturwissenschaftlich-Mathematischen Gesamtfakultät der Ruprecht-Karls-Universität Heidelberg vorgelegt von Hélio Roque Tag der mündlichen Prüfung: DISSERTATION Submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by Hélio Roque Date of oral examination: A closer look at Schizosaccharomyces pombe interphase microtubule arrays Referees: Damian Brunner David Robinson Summary Determination of cell shape is an essential mechanism for cells like neurons, epithelial and fungal. In general, these cells are able to control their shape by positioning mechanisms that regulate cell growth and cell polarity. One key element for such a process is the microtubule cytoskeleton, which is organized into higher order assemblies in polarized cells. Therefore, an important question is to understand how these assemblies are established and maintained during interphase. We addressed this question by making use of mutants of the fission yeast Schizosaccharomyces pombe to study the organization of Interphase Microtubule Assemblies in high ultra-structural detail, using a new emerging technology – electron tomography.